The ACE Pico Protocol introduces a transformative platform in regenerative medicine and pharmaceutical biotechnology by converting stem cells into polydisperse nanoscale fragments (5\u201320 nm) through a cold (<10\u00b0C), mechanical dissociation process. This protocol enables non-invasive, multible-doses sublingual administration of a “whole cell” payload\u2014including membranes, organelles, proteins, and nucleic acids\u2014addressing longstanding challenges of multi-dosing, immune response, and patient compliance in cellular therapies.<\/p>\n
Comprehensive analysis using electron microscopy (EM) and UV-Vis spectrophotometry confirmed the bioactive integrity of the processed materials, with strong absorbance peaks for proteins and pigments, and optimal particle sizes for mucosal uptake. In animal models, high-dose sublingual administration (2000 mg\/kg) revealed no toxicity, while plasma protein studies across five administration routes demonstrated the highest systemic protein increase via the sublingual route (0.66 g\/dL, p<0.01). These findings underscore superior absorption, therapeutic potential, and safety.<\/p>\n
Compared to exosomes, peptides, or liposomal delivery systems, the ACE Pico Protocol offers broader therapeutic payloads, reduced degradation risk, and enhanced stability, making it a compelling platform for next-generation pharmaceutical formulations and biologic delivery systems.<\/p>\n
Authors:<\/p>\n
Dr. Alaa Abdelkarim Fouad , ACE Cells Lab Ltd, UK<\/p>\n
Keywords: ACE Pico Protocol, nanoscale delivery, stem cell therapy, sublingual administration, pharmaceutical innovation, regenerative medicine, multi-dosing challenge<\/p>\n","protected":false},"excerpt":{"rendered":"
The ACE Pico Protocol introduces a transformative platform in regenerative medicine and pharmaceutical biotechnology by converting stem cells into polydisperse nanoscale fragments (5\u201320 nm) through a cold (<10\u00b0C), mechanical dissociation process. This protocol enables non-invasive, multible-doses sublingual administration of a “whole cell” payload\u2014including membranes, organelles, proteins, and nucleic acids\u2014addressing longstanding challenges of multi-dosing, immune response, […]<\/p>\n","protected":false},"featured_media":5375,"template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","_seopress_news_disabled":"","_seopress_video_disabled":"","_seopress_video":[],"_seopress_pro_schemas_manual":[],"_seopress_pro_rich_snippets_disable_all":"","_seopress_pro_rich_snippets_disable":[],"_seopress_pro_schemas":[],"_activity_time_start":"15:30","_activity_time_end":"15:50","_activity_date":"2025-05-22","_activity_price":"0","footnotes":""},"edition":[36],"location":[],"track":[27],"activity_type":[17],"class_list":["post-6836","activity","type-activity","status-publish","has-post-thumbnail","hentry","edition-chemuk-2025","track-27","activity_type-full-programme"],"_guests":[{"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/guest\/3862"}],"_sponsors":[],"_cover_image":null,"_links":{"self":[{"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/activity\/6836","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/activity"}],"about":[{"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/types\/activity"}],"version-history":[{"count":0,"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/activity\/6836\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/media\/5375"}],"wp:attachment":[{"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/media?parent=6836"}],"wp:term":[{"taxonomy":"edition","embeddable":true,"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/edition?post=6836"},{"taxonomy":"location","embeddable":true,"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/location?post=6836"},{"taxonomy":"track","embeddable":true,"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/track?post=6836"},{"taxonomy":"activity_type","embeddable":true,"href":"https:\/\/www.chemicalukexpo.com\/en\/wp-json\/wp\/v2\/activity_type?post=6836"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}